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high stringency wash buffer  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology high stringency wash buffer
    High Stringency Wash Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 11767 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+buffer/Triton+X-100/pm37169843-399-43-24
    Average 96 stars, based on 11767 article reviews
    high stringency wash buffer - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: Functional Roles of Sex-Biased, Growth Hormone–Regulated MicroRNAs miR-1948 and miR-802 in Young Adult Mouse Liver
    Article Snippet: The beads were then washed three times with 1 mL of 1× PXL buffer, twice with 1 mL high-salt buffer (1× PBS, 1 M NaCl, 1% Ipegal, 0.5% sodium deoxycholate, 0.1% SDS) (Santa Cruz Biotechnology, Santa Cruz, CA), twice with 1 mL of high-stringency buffer (1 mM Tris-HCl pH 7.5, 5 mM EDTA, 2.5 mM EGTA, 1% Ipegal, 1% sodium deoxycholate, 0.1% SDS, 120 mM NaCl, 25 mM KCl), and once with 1 mL of low-salt wash buffer (15 mM Tris pH 7.5, 5 mM EDTA).

    Article Title: A sense-antisense RNA interaction promotes breast cancer metastasis via regulation of NQO1 expression.
    Article Snippet: The clarified lysate was transferred to protein A Dynabeads conjugated to anti-HNRNPC (5 μg antibody per confluent 15-cm plate of cells, catalog no. sc-32308, Santa Cruz Biotechnology) and rotated end-over-end at 4 °C for 2 h. The beads were then washed once with high-stringency wash buffer (15 mM Tris pH 7.5, 5 mM EDTA, 1% Triton X-100, 1% sodium deoxycholate, 0.001% SDS, 120 mM NaCl, 25 mM KCl), once with high-salt wash buffer (15 mM Tris pH 7.5, 5 mM EDTA, 1% Triton X-100, 1% sodium deoxycholate, 0.001% SDS, 1 M NaCl) and once with ×1 PBS.

    Article Title: Functional interaction between cellular p100 and the dengue virus 3' UTR.
    Article Snippet: Immune complexes were precipitated with a slurry of protein A–agarose (Santa Cruz) and washed five times with high stringency RIP assay buffer (50 mM Tris/ HCl, pH 7.5, 1 % Nonidet P-40, 1 % sodium deoxycholate, 0.1 % SDS, 1 mM EDTA, 1 M NaCl and 0.2 mM PMSF).



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